Laboratory Test Preparation

Test

Coenzyme Q10 (HPLC)

Other Test Request Name

• Ubiquinone
• Coenzyme Q10

Test Composition

Not applicable

Intended Use

This test is used to check the blood level of CoQ10 and will enable more precise dosing for anyone seeking to achieve and maintain high levels of this critical antioxidant.Coenzyme Q10 is produced by the human body and is necessary for the basic functioning of all cells. It is known to be highly concentrated in heart muscle cells due to the high energy requirements of this cell type.

Methodology

High Performance Liquid Chromatography (HPLC)

Laboratory Section

Special Test

Special Instructions/Patient Preparations

No patient preparation necessary.

Collection/Sample Container

Red tube (DO NOT USE GOLD TUBE or any other type of tube with gel separator)

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.

4 mL Serum

Alternative Specimen and Volume Requirement

Not Applicable

Specimen Stability

•   Room Temperature (15-25°C)

24 Hours

•   Refrigerated Temperature (2-8°C)

7 Days

•   Freezer Temperature (-20°C)

28 Days

Transport Temperature

Transport specimen at 2°C - 8°C (with cold packs)

Rejection Criteria

• Hemolyzed specimen
• Quantity Not sufficient
• Markedly lipemic sample
• Improperly labeled specimen

Running Day

Batch running

Cut Off Time

Friday, 4:00 PM

TAT/Releasing of Results

1 month after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation

Available upon request

Limitations/Interferences

None specified

Frequently Asked Questions (FAQs)

Not applicable

Related Words/Test

Disease, low dietary intake, or high CoQ10 use by the body

Test

Cold Agglutinins-Temporarily Unavailable (currently not accepting samples)

Other Test Request Name

Cold Hemagglutinins

Test Composition

Not applicable

Intended Use

Useful for the detection of cold agglutinins in association with cold agglutinin syndrome

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Methodology

Hemagglutination (HA)

Laboratory Section

Special Test

Special Instructions/Patient Preparations

Do not use tube with Gel Separator

Collection/Sample Container

Red Top Only

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.

3 mL Serum

Alternative Specimen and Volume Requirement

Not Applicable

Specimen Stability

•   Room Temperature (15-25°C)

7 Days

•   Refrigerated Temperature (2-8°C)

14 Days

•   Freezer Temperature (-20°C)

Not applicable

Transport Temperature

Transport specimen at 2°C - 8°C (with cold packs)

Rejection Criteria

• Hemolyzed specimen
• Quantity Not sufficient
• Markedly lipemic sample
• Improperly labeled specimen

Running Day

Batch running

Cut Off Time

Monday, 12:00 PM

TAT/Releasing of Results

3 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation

Available upon request

Limitations/Interferences

None specified

Frequently Asked Questions (FAQs)

Not applicable

Related Words/Test

Agglutinins; Cold Autoantibodies; Cold-Reacting Antibodies, Cold Agglutinins

Test

Complement C2

Other Test Request Name

• Complement - C2 fraction
• C2 fraction - complement
• C2 complement fraction

Test Composition

Not applicable

Intended Use

Component C2 is involved in the classic complement activation pathway. After cleavage by C1s, C2 enters into the composition of the C3 and C5 convertases.Homozygous C2 deficiency is the most common of the full congenital complement component deficiencies and half of the subjects thus affected present with a lupus-like syndrome with, in some cases, renal involvement or recurrent infections.

Methodology

Radial Immunodiffusion

Laboratory Section

Special Test

Special Instructions/Patient Preparations

No patient preparation necessary.

Collection/Sample Container

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.

3 mL Serum

Alternative Specimen and Volume Requirement

Not Applicable

Specimen Stability

•   Room Temperature (15-25°C)

24 Hours

•   Refrigerated Temperature (2-8°C)

7 Days

•   Freezer Temperature (-20°C)

28 Days

Transport Temperature

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria

• Hemolyzed specimen
• Quantity Not sufficient
• Improperly labeled specimen
• Improperly collection tube used

Running Day

Batch Running

Cut Off Time

Friday, 4:00 PM

TAT/Releasing of Results

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation

Available upon request

Limitations/Interferences

None specified

Frequently Asked Questions (FAQs)

Not Applicable

Related Words/Test

C1; C1q; C2; C3; C4; CH50; CH100, Complement Activity; Complement Component; Total Complement; Total Hemolytic Complement Activity

Test

Complement C3

Other Test Request Name

• C3

• Complement Component 3

Test Composition

Not applicable

Intended Use

• Aids in the diagnosis of immunologic disorders, especially those associated with deficiencies of complement components.
• Help diagnose the cause of recurrent microbial infections (such as Streptococcus pneumoniae, Neisseria meningitides, Neisseria gonorrhea), angioedema, or inflammation.
• Help diagnose and monitor the activity and treatment of acute or chronic autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis.
• Monitor immune complex-related diseases and conditions such as glomerulonephritis (a kidney disorder), serum sickness, and vasculitis.

Methodology

Immunoturbidimetry

Laboratory Section

Chemistry

Special Instructions/Patient Preparations

• No patient preparation necessary.

Collection/Sample Container

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.

1-3 mL Serum

Alternative Specimen and Volume Requirement

1-3mL Plasma (Heparin)

Specimen Stability

•   Room Temperature (15-25°C)

1 Day

•   Refrigerated Temperature (2-8°C)

3 Days

•   Freezer Temperature (-20°C)

1 Month

Transport Temperature

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria

• Improper collection tube used
• Specimen stored/transported outside the temperature range
• Quantity not sufficient
• Hemolyzed specimen
• Lipemic specimen
• Improperly labeled specimen
• Plasma from EDTA tube

Running Day

Monday, Wednesday, Friday

Cut Off Time

Monday to Saturday:6:00 PM

Sunday:4:00PM

TAT/Releasing of Results

ROUTINE (on running days)
• 4 hours after receipt of specimen/ arrival of messenger
STAT (on running days only)
• After 2½ HOURS from extraction/messenger arrival

Reference Interval/Result Interpretation

1 Day to 14 Y/O MALE: 800-1700 mg/L (80-170 mg/dL)

1 Day to 14 Y/O FEMALE: 820-1730 mg/L (82-173 mg/dL)

14Y & 1 Day to 999 Y/O MALE: 820-1730 mg/L (82-173 mg/dL)

14Y & 1 Day to 999 Y/O FEMALE: 830-1930 mg/L (83-193 mg/dL)

Limitations/Interferences

None identified.

Frequently Asked Questions (FAQs)

Not applicable

Related Words/Test

ES,C-Reactive Protein, Rheumatoid Factor, ANA, Autoantibodies

Test

Complement C4

Other Test Request Name

• C4
• Complement Component 4

Test Composition

Not applicable

Intended Use

• C4 measurement aids in the diagnosis of immunologic disorders, especially those associated with deficiencies of complement components and to assist in the diagnosis of immunological diseases, such as rheumatoid arthritis, and systemic lupus erythematosus (SLE), in which complement is consumed at an increased rate, or to detect inborn deficiency.

Methodology

Immunoturbidimetry

Laboratory Section

Chemistry

Special Instructions/Patient Preparations

• No patient preparation necessary.
• Centrifuge specimen and remove serum or plasma from the cellular material within two hours of collection.

Collection/Sample Container

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.

1-3 mL Serum

Alternative Specimen and Volume Requirement

1-3mL Plasma (Heparin)

Specimen Stability

•   Room Temperature (15-25°C)

Not specified

•   Refrigerated Temperature (2-8°C)

2 Days

•   Freezer Temperature (-20°C)

Not specified

Transport Temperature

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria

• Improper collection tube used
• Specimen stored/transported outside the temperature range
• Quantity not sufficient
• Hemolyzed specimen
• Lipemic specimen
• Improperly labeled specimen

Running Day

Wednesday

Cut Off Time

Monday to Saturday:6:00 PM

Sunday:4:00PM

TAT/Releasing of Results

RUNNING TIME: 3:00PM onwards on running day/s

RELEASING TIME: 4:00PM onwards on running day/s

Reference Interval/Result Interpretation

0-14Y MALE: 140~440 mg/L (14-44 mg/dL)
0-14Y FEMALE: 130~460 mg/L (13-46 mg/dL)
14Y & 1 Day-999Y MALE: 150~530 mg/L (15~53 mg/dL)
14Y & 1 Day-999Y FEMALE: 150~570 mg/L (15-57 mg/dL)

Limitations/Interferences

None identified.

Frequently Asked Questions (FAQs)

Not applicable

Related Words/Test

ES,C-Reactive Protein, Rheumatoid Factor, ANA, Autoantibodies

Test

Complement CH50

Other Test Request Name

Total haemolytic complement

Test Composition

Not Applicable

Intended Use

A functional assay of total classic complement pathway activity. An abnormal CH50 result should be followed up by measuring C3 and C4 levels. A high result indicates an inflammatory syndrome and a low result is suggestive of deficiency due to reduced synthesis, either congenital or acquired.

Methodology

Analytical immunology

Laboratory Section

Special Test

Special Instructions/Patient Preparations

No patient preparation necessary.

Collection/Sample Container

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.

3 mL Serum

Alternative Specimen and Volume Requirement

Not Applicable

Specimen Stability

•   Room Temperature (15-25°C)

Not applicable

•   Refrigerated Temperature (2-8°C)

1 Hour

•   Freezer Temperature (-20°C)

28 Days

Transport Temperature

Transport specimen at 2°C - 8°C (with cold packs)

Rejection Criteria

• Hemolyzed specimen
• Quantity Not sufficient
• Markedly lipemic sample
• Improperly labeled specimen

Running Day

Batch Running

Cut Off Time

Friday, 4:00 PM

TAT/Releasing of Results

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation

Not Applicable

Limitations/Interferences

None Specified

Frequently Asked Questions (FAQs)

Not Applicable

Related Words/Test

C1; C1q; C2; C3; C4; CH50; CH100, Complement Activity; Complement Component; Total Complement; Total Hemolytic Complement Activity

Test

Complete Blood Count.

Other Test Request Name

Full blood count, CBC, hemogram, CBC with Differential (CBC with diff)

Test

Complete Urinalysis

Other Test Request Name

•Random Urinalysis
• Spot Urinalysis
• Urine Test
• Dipstick Test
• U/A

Test Composition

Macroscopic Test
• Color
• Transparency
Chemical Test
• Specific Gravity
• pH
• Glucose
• Protein
• Nitrite
• Leukocyte Esterase
• Bilirubin
• Blood (Ery/Hgb)
• Urobilinogen
• Ketone
Microscopic Test
• RBC
• WBC
• Epithelial cells
• Bacteria
• Mucus Threads
• Amorphous Urates/ phosphates
• Crystal/s
• Cast/s

Intended Use

• For the detection of substances or cellular material in the urine associated with different metabolic and kidney disorders.
• It is a good test for assessing the overall health of a patient

Methodology

Chemical Urine test
• Reagent Impregnated Strips
Automated Microscopic Test
• Fluorescence Flow Cytometry with Hydrodynamic Focusing
Microscopic Manual Urine Test
• Direct Microscopic Examination

Laboratory Section

Clinical Microscopy

Special Instructions/Patient Preparations

• Patient is advised to collect first morning or random midstream clean-catch urine sample. “Midstream collection” is performed by passing the first portion of the urine into the bedpan or toilet and collecting the midportion in the appropriate specimen container without contaminating the container.
• For infants and young children, urine specimen collection bags can be used to collect specimen.
• For patients with monthly period, collect sample minimum of 3 days after the last day of menstruation.

Collection/Sample Container

Clean, leak-proof container

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.

10-15 mL Urine

NOTE: For pediatric and geriatric patients, minimun of 5 mL is acceptable.

Alternative Specimen and Volume Requirement

Not applicable

Specimen Stability

•   Room Temperature (15-25°C)

2 Hours

•   Refrigerated Temperature (2-8°C)

24 Hours

•   Freezer Temperature (-20°C)

Not applicable

Transport Temperature

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria

• Quantity not sufficient (QNS): Samples less than 10mL
NOTE: For pediatric and geriatric patients, minimun of 5 mL is acceptable.
• Specimen with fecal contamination
• Decomposition or bacterial overgrowth due to delayed transport of urine
• Improperly labeled sample
• Patient collected urine sample during menstruation OR less than 3 days of last menstruation.

Running Day

Daily

Cut Off Time

Monday to Saturday
• 6:00 PM

Sunday
• 3:00 PM

TAT/Releasing of Results

ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• After 1½ hours from receipt of specimen/ arrival of messenger

Reference Interval/Result Interpretation

Available upon request.

Limitations/Interferences

BLOOD:
Results may be affected by RBC lysis, high concentrations of ascorbic acid, nitrites and protein, dehydration, exercise, microbial peroxidase activities in UTI.
LEUKOCYTES:
Results may be affected by factors like massive bacteriuria, increase protein, glucose, blood, highly colored urine, high doses of medicines like gentamicin, tetracycline, cephalexin, and contamination with secretions.

Frequently Asked Questions (FAQs)

Q: What is the difference of URINUF AND URINAL?
A: URINUF test code is used if clients wants microscopic reading using automation while URINAL test code is used if clients want manual microscopic reading.
Q: If patient is with menstruation, can she still proceed with urinalysis?
A: NO she is ONLY allowed to proceed with the urinalysis if her menstruation is 3 days after her last day of menstruation.
Q: How long can we stand the urine after collection?
A: Stability of urine is only 2 hours at room temperature (15-25°C). In case of delayed processing, specimen should be refrigerated at 2°C~8°C.

Related Words/Test

UTI, Urine Culture, Urine Protein, BUN, Creatinine, Creatinine Clearance, Urine Albumin, eGFR, Kidney Stone Analysis, Glucose Tests, Renal Panel, Bilirubin

Test

Compre. Leukemia Panel (BY APPOINTMENT)

Other Test Request Name

Not applicable

Test Composition

Not Applicable

Intended Use

To aid in the diagnosis, lineage assignment, subclassification, and response to treatment of hematologic malignancies.

Methodology

Flow Cytometry

Laboratory Section

Special Test

Special Instructions/Patient Preparations

• No patient preparation necessary.
• Request Container to Special test section
• Call Special Test Section (By Appointment/Schedule).
• Request Container to Special test section
• Need Clinical Abstract

NOTE: Do not collect specimen without approval of Special Test Section

Collection/Sample Container

Sodium Heparin Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.

Peripheral Blood - 4 pcs of 4mL Sodium Heparin whole blood and

2 pc of 4mL EDTA whole blood

Alternative Specimen and Volume Requirement

Bone Marrow Aspirate - 3pcs of 4mL Sodium Heparin and 2 unstained smear (direct smear)

Specimen Stability

•   Room Temperature (15-25°C)

24 Hours

•   Refrigerated Temperature (2-8°C)

Not Applicable

•   Freezer Temperature (-20°C)

Not Applicable

Transport Temperature

Transport specimen at 15°C ~25 °C (room temperature)

Rejection Criteria

• Improperly labeled specimens
• Clotted specimens
• Over-filled or Under-filled tube
• Quantity not sufficient
• Specimen stored and transported outside the required temperature

Running Day

Monday to Friday

Cut Off Time

Monday to Friday, 7:00 AM

TAT/Releasing of Results

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation

Not Applicable

Limitations/Interferences

None Specified

Frequently Asked Questions (FAQs)

Not Applicable

Related Words/Test

Not Applicable

Test

Concentration Technique

Other Test Request Name

Stool concentration

Test Composition

Not applicable

Intended Use

Aids in early detection of parasitic infection by fecal parasite concentration for the detection of helminth ova and larvae/ protozoa cysts and oocysts.

Methodology

Direct Microscopic Examination

Laboratory Section

Clinical Microscopy

Special Instructions/Patient Preparations

No patient preparation necessary.

Collection/Sample Container

Clean, leak-proof container

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.

Pea sized or size of 25 centavo coin stool

Alternative Specimen and Volume Requirement

Not applicable

Specimen Stability

•   Room Temperature (15-25°C)

Liquid: 30 Minutes
Soft: 30 Minutes
Semi-formed: 1 Hour
Formed: 24 Hours

•   Refrigerated Temperature (2-8°C)

Not specified

•   Freezer Temperature (-20°C)

Unacceptable

Transport Temperature

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria

• Quantity not sufficient (QNS); samples less than pea-sized stool
• Contaminated stool (e.g. with urine or other body fluids)
• Stool not delivered immediately in the laboratory within 2 hours.

Running Day

Daily

Cut Off Time

Monday to Saturday
• 6:00 PM
Sunday
• 3:00 PM

TAT/Releasing of Results

ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• After 1½ hours from receipt of specimen/ arrival of messenger

Reference Interval/Result Interpretation

NO INTERTINAL PARASITE SEEN

Limitations/Interferences

Contaminated stool sample may interfere with the results.

Frequently Asked Questions (FAQs)

Not applicable

Related Words/Test

Amoebiasis, diarrhea, parasitic infection

Test

Confirmatory Test (METH)

Other Test Request Name

Not applicable

Test Composition

Not applicable

IntendedUse

Used as a confirmatory test for qualitative and quantitative detection of methamphetamine in urine.

Methodology

Gas Chromatography-Mass Spectrometry

Laboratory Section

Drug Test

Special Instructions/Patient Preparations

No patient preparation necessary

Collection/Sample Container

60-mL clean, screw cap, polyethylene container

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.

60 mL urine

Alternative Specimen and Volume Requirement

Not applicable

Specimen Stability

•   Room Temperature (15-25°C)

Not specified

•   Refrigerated Temperature (2-8°C)

48 Hours

•   Freezer Temperature (-20°C)

Not specified

Transport Temperature

Not applicable

Rejection Criteria

• Incompatibility of the ID number on the specimen received by the laboratory with the number on the CCF.
• Absence of ID number on the specimen
• No printed Authorized Specimen Collector’s name and signature on the CCF.
• Broken or tampered seal on the specimen container
• Insufficient quantity of specimen, below 60 mL for single collection; below 30 mL for spilt collection

Running Day

Daily

Cut Off Time

Monday to Saturday
• 6:00 PM
Sunday
• 3:00 PM

TAT/Releasing of Results

2 to 3 weeks upon issuance to confirmatory laboratory

Reference Interval/Result Interpretation

Not applicable

Limitations/Interferences

Some prescription medications contain amphetamine or methamphetamine, while the active ingredient of some prescription medication may be metabolized to amphetamine or methamphetamine, causing positive amphetamine/methamphetamine test results

Frequently Asked Questions (FAQs)

Not applicable

Related Words/Test

Not applicable

Test

Crea Clearance

Other Test Request Name

• Estimated Creatinine Clearance
• Creatinine Clearance

Test Composition

1. Creatinine
2. Creatinine Clearance

Intended Use

Usually used in clinical research studies to evaluate kidney function of patient.

Methodology

Equations used in determining estimated crea clearance differ from one study to another.

Laboratory Section

Chemistry

Special Instructions/Patient Preparations

• Refrain from eating a high meat diet and doing strenuous physical activity 8-12 hours before blood extraction.
• Refrain from taking medicines such as Acetaminophen (Paracetamol), N-acetyl-p-benzoquinone imine (NAPQI) metabolites, N-Acteylcysteine (NAC), Metamizole (Novaminsulfone, Dipyrone), 4-aminoantipyrine (4-AAP) and 4-Methylamino-antipyrine (4-MAP) metabolites 8-12 hrs before sample collection.

Note: If recommended preparation is not followed, you may accept but NOTE in clinical info.

Collection/Sample Container

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.

1-3 mL Serum

Alternative Specimen and Volume Requirement

1-3mL Plasma (Li?heparin and K2?EDTA)

Specimen Stability

•   Room Temperature (15-25°C)

7 Days

•   Refrigerated Temperature (2-8°C)

7 Days

•   Freezer Temperature (-20°C)

3 Months

Transport Temperature

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day

Daily

Cut Off Time

Monday to Saturday:10:00 PM

Sunday:6:00PM

TAT/Releasing of Results

RUNNING TIME: 6:00 AM onwards

RELEASING TIME: ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
RELEASING TIME: STAT
• After 2½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation

CREATININE:
0-2 Months: 27.00-77.00 umol/L (0.31-0.87 mg/dL)
2M & 1 Day - 1Y: 14.00-34.00 umol/L (0.16-0.38 mg/dL)
1Y & 1 Day - 3Y: 15.00-31.00 umol/L (0.17-0.35 mg/dL)
3Y & 1 Day - 5Y: 23.00-37.00 umol/L (0.26-0.42 mg/dL)
5Y & 1 Day - 7Y: 25.00-42.00 umol/L (0.28-0.47 mg/dL)
7Y & 1 Day - 9Y: 30.00-47.00 umol/L (0.34-0.53 mg/dL)
9Y & 1 Day - 11Y: 29.00-56.00 umol/L (0.33-0.63 mg/dL)
11Y & 1 Day - 13Y: 39.00-60.00 umol/L (0.44-0.68 mg/dL)
13Y & 1 Day - 15Y: 40.00-68.00 umol/L (0.45-0.77 mg/dL)
15Y & 1 Day - 999Y MALE: 59.00-104.00 umol/L (0.67-1.18 mg/dL)
15Y & 1 Day - 999Y FEMALE: 45.00-84.00 umol/L (0.51-0.95 mg/dL)

Limitations/Interferences

Rifampicin, Levodopa and Calcium dobesilate (e.g. Dexium) cause artificially low creatinine results. Dicynone (Etamsylate) at therapeutic concentrations may lead to falsely low results.
• N?ethylglycine at therapeutic concentrations and DL?proline at concentrations ≥ 1 mmol/L (≥ 115 mg/L) give falsely high results.
• 2?Phenyl?1,3?indandion (Phenindion) at therapeutic concentrations interferes with the assay.
• In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s macroglobulinemia), may cause unreliable results.
• Acetaminophen intoxications are frequently treated with N?Acetylcysteine. N?Acetylcysteine at a plasma concentration above 333 mg/L and the Acetaminophen metabolite N?acetyl?p?benzoquinone imine (NAPQI) independently may cause falsely low results.
• Venipuncture should be performed prior to the administration of Metamizole. Venipuncture immediately after or during the administration of Metamizole may lead to falsely low results. A significant interference may occur at any plasma Metamizole concentration

Frequently Asked Questions (FAQs)

Not applicable

Related Words/Test

Kidney Function

Test

Creatinine (ENZYMATIC)

Other Test Request Name

• Creat
• Blood Creatinine
• Serum Creatinine

Test Composition

Not applicable

Intended Use

The assay of creatinine in serum or plasma is the most commonly used test to assess renal function.

Methodology

Enzymatic

Laboratory Section

Chemistry

Special Instructions/Patient Preparations

• Refrain from eating a high meat diet and doing strenuous physical activity 8-12 hours before blood extraction.
• Refrain from taking medicines such as Acetaminophen (Paracetamol), N-acetyl-p-benzoquinone imine (NAPQI) metabolites, N-Acteylcysteine (NAC), Metamizole (Novaminsulfone, Dipyrone), 4-aminoantipyrine (4-AAP) and 4-Methylamino-antipyrine (4-MAP) metabolites 8-12 hrs before sample collection.

Note: If recommended preparation is not followed, you may accept but NOTE in clinical info.

Collection/Sample Container

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.

1-3 mL Serum

Alternative Specimen and Volume Requirement

1-3mL Plasma (Li-heparin and K2-EDTA)

Specimen Stability

•   Room Temperature (15-25°C)

7 Days

•   Refrigerated Temperature (2-8°C)

7 Days

•   Freezer Temperature (-20°C)

3 Months

Transport Temperature

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria

• Specimens that failed the serum index criteria
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day

Daily

Cut Off Time

Monday to Saturday:  6:00 PM

Sunday: 4:00 PM

TAT/Releasing of Results

ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
STAT
• After 2½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation

0-2 Months: 27.00-77.00 umol/L (0.31-0.87 mg/dL)
2M & 1 Day - 1Y: 14.00-34.00 umol/L (0.16-0.38 mg/dL)
1Y & 1 Day - 3Y: 15.00-31.00 umol/L (0.17-0.35 mg/dL)
3Y & 1 Day - 5Y: 23.00-37.00 umol/L (0.26-0.42 mg/dL)
5Y & 1 Day - 7Y: 25.00-42.00 umol/L (0.28-0.47 mg/dL)
7Y & 1 Day - 9Y: 30.00-47.00 umol/L (0.34-0.53 mg/dL)
9Y & 1 Day - 11Y: 29.00-56.00 umol/L (0.33-0.63 mg/dL)
11Y & 1 Day - 13Y: 39.00-60.00 umol/L (0.44-0.68 mg/dL)
13Y & 1 Day - 15Y: 40.00-68.00 umol/L (0.45-0.77 mg/dL)
15Y & 1 Day - 999Y MALE: 59.00-104.00 umol/L (0.67-1.18 mg/dL)
15Y & 1 Day - 999Y FEMALE: 45.00-84.00 umol/L (0.51-0.95 mg/dL)

Limitations/Interferences

• Rifampicin, Levodopa and Calcium dobesilate (e.g. Dexium) cause artificially low creatinine results. Dicynone (Etamsylate) at therapeutic concentrations may lead to falsely low results.
• N-ethylglycine at therapeutic concentrations and DL-proline at concentrations ≥ 1 mmol/L (≥ 115 mg/L) give falsely high results.
• 2-Phenyl-1,3-indandion (Phenindion) at therapeutic concentrations interferes with the assay.
• In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s macroglobulinemia), may cause unreliable results.
• Acetaminophen intoxications are frequently treated with N?Acetylcysteine. N-Acetylcysteine at a plasma concentration above 333 mg/L and the Acetaminophen metabolite N-acetyl-p-benzoquinone imine (NAPQI) independently may cause falsely low results.
• Venipuncture should be performed prior to the administration of Metamizole. Venipuncture immediately after or during the administration of Metamizole may lead to falsely low results. A significant interference may occur at any plasma Metamizole concentration.

Frequently Asked Questions (FAQs)

Not applicable

Related Words/Test

BUN, eGFR, Creatinine Clearance, Comprehensive Metabolic Panel, Basic Metabolic Panel, Urinalysis, Urine Protein to Creatinine Ratio, Urine Albumin and Urine Albumin/Creatinine Ratio, Renal Panel, Cystatin C, Beta-2 Microglobulin

Test

CRP (C-Reactive Protein)

Other Test Request Name

C-Reactive Protein

CRP

Test Composition

Not Applicable

Intended Use

To identify the presence of inflammation and to monitor response to treatment for an inflammatory disorder

Methodology

Turbidimetric/ Immunoturbidimetric

Laboratory Section

Chemistry

Special Instructions/Patient Preparations

No patient preparation necessary.

Collection/Sample Container

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.

1-3mL Serum

Alternative Specimen and Volume Requirement

1-3 mL Plasma (Li- or Na-Heparin, EDTA)

Specimen Stability

•   Room Temperature (15-25°C)

15 Days

•   Refrigerated Temperature (2-8°C)

2 Months

•   Freezer Temperature (-20°C)

3 Years

Transport Temperature

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria

• Failed serum index specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day

Daily

Cut Off Time

Monday to Saturday: 6:00 PM
Sunday: 4:00 PM

TAT/Releasing of Results

• ROUTINE (on running days): 4 hours after receipt of specimen/ arrival of messenger
• STAT (on running days only): After 2 1/2 hours from extraction/ messenger arrival

Reference Interval/Result Interpretation

• </= 5.00 mg/L NEGATIVE
• >5.00 mg/L POSITIVE

Limitations/Interferences

Contaminated and markedly lipemic sera may cause non-specific reactions and should therefore not be tested.

Frequently Asked Questions (FAQs)

Q: What is the difference between CRP and hs-CRP tests?
A: Both tests are essentially the same, measuring the same substance in the blood. However, the high sensitivity CRP (hs-CRP) test measures very small amounts of CRP in the blood and is ordered most frequently for seemingly healthy people to assess their potential risk for heart problems. The regular CRP test is ordered for those at risk for infections or chronic inflammatory diseases

Related Words/Test

Inflammation, Acute Phase Reactant

Test

Cryptosporidium Test

Other Test Request Name

Crypto

Test Composition

Not Applicable

Intended Use

Intestinal parasite that affects healthy human and animals, and cause an unrelenting infection in immuno compromised individuals such as AIDs patient

Cryptosporidium is a genus of apicomplexan parasitic alveolates that can cause a respiratory and gastrointestinal illness that primarily involves watery diarrhea with or without a persistent cough in both immunocompetent and immunodeficient humans.

Methodology

Modified AFB Stain

Laboratory Section

Special Test

Special Instructions/Patient Preparations

• No patient preparation necessary.
• Sample must be at Laboratory, Monday to Friday before 7:00 AM.
• Sample must be collected Monday to Thursday, on or before 3:00 PM and need to be send to MAIN branch within that day.

Collection/Sample Container

Sterile leak proof container

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.

20g Stool/Feces

Alternative Specimen and Volume Requirement

Not Applicable

Specimen Stability

•   Room Temperature (15-25°C)

24Hours

•   Refrigerated Temperature (2-8°C)

24Hours

•   Freezer Temperature (-20°C)

Not Applicable

Transport Temperature

Transport specimen at 2°C - 8°C (with cold packs)

Rejection Criteria

• Quantity Not Sufficient
• Improperly labeled specimen
• Stool in preservative

Running Day

Monday to Friday

Cut Off Time

Monday to Friday, 7:00 AM

• Sample must be collected Monday to Thursday, on or before 3:00 PM and need to be send to MAIN branch within that day.

TAT/Releasing of Results

3 days after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation

Available upon request

Limitations/Interferences

None Specified

Frequently Asked Questions (FAQs)

Not Applicable

Related Words/Test

Not Applicable