Test
Anti HMGCR
Other Test Request Name
Test Composition
Not applicable
Intended Use
They have been implicated in the occurrence of acquired inflammatory myopathies related to the existence of anti-HMGCR antibodies. The assay of anti-HMGCR antibodies (also present in 50% of thse not receiving statins) allows the diagnosis of necrotising autoimmune myopathy and commencing an effective immunosuppressant treatment.
Methodology
Chemiluminescence
Laboratory Section
Special Test
Special Instructions/Patient Preparations
No patient preparation necessary.
Collection/Sample Container
Red or Gold tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
3 mL Serum
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
24 Hours
• Refrigerated Temperature (2-8°C)
7 Days
• Freezer Temperature (-20°C)
28 Days
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
Running Day
Batch Running
Cut Off TimeFriday, 4:00 PM
TAT/Releasing of Results
3 months after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation
Available upon request
Limitations/Interferences
None specified
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
Necrotizing Autoimmune Myopathy (NAM)
Test
Anti Mitochondrial Ab (IF)
Other Test Request Name
Test Composition
Not Applicable
Intended Use
This test detects the presence of antibodies against mitochondria, which is useful in the diagnosis of Primary Biliary Cirrhosis (PBC).
Methodology
Indirect Immunofluorescence Test
Laboratory Section
Serology
Special Instructions/Patient Preparations
No Patient preparation required.
Collection/Sample Container
Plain Red Top or Gold Top.
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
2 - 3 mL Serum
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
Not specified
• Refrigerated Temperature (2-8°C)
7 days
• Freezer Temperature (-20°C)
2 Weeks
Transport Temperature
Trasport specimen at 2ºC to 10ºC (with cold packs)
Rejection Criteria
Running DayWednesday
Cut Off Time
Tuesday, 8:00PM
TAT/Releasing of Results
Thursday, 4:00PM
Reference Interval/Result Interpretation
Significant Level:
Limitations/Interferences
None Identified
Frequently Asked Questions (FAQs)
Not Applicable
Related Words/Test
Test
Anti MOG (IF)
Other Test Request Name
Anti-Myelin Oligodendrocyte
Test Composition
Not applicable
Intended Use
Myelin Oligodendrocyte glycoprotein (MOG) is a key CNS-specific autoantigen for primary demyelination in multiple sclerosis.
Methodology
Immunofluorescence
Laboratory Section
Special Test
Special Instructions/Patient Preparations
No patient preparation necessary.
Collection/Sample Container
Red or Gold tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
3 mL Serum
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
24 Hours
• Refrigerated Temperature (2-8°C)
7 Days
• Freezer Temperature (-20°C)
28 Days
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
Running Day
Batch Running
Cut Off Time
Friday, 4:00 PM
TAT/Releasing of Results
1 month after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation
Available upon request
Limitations/Interferences
None specified
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
Inflammatory demyelinating disease of the central nervous system, neuromyelitis optica spectrum disorder
Test
Anti MPO(Myeloperoxidase)
Other Test Request Name
Test Composition
Not applicable
Intended Use
Anti-ANCA Ab represents a recently developed marker which is very useful in the diagnosis of vasculitis.
Methodology
Flow Immunofluorimetry
Laboratory Section
Special Test
Special Instructions/Patient Preparations
Collection/Sample Container
Red or Gold tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
3 mL Serum
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
24 Hours
• Refrigerated Temperature (2-8°C)
7 Days
• Freezer Temperature (-20°C)
30 Days
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
Running Day
Batch Running
Cut Off Time
Friday, 4:00 PM
TAT/Releasing of Results
2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation
Available upon request
Limitations/Interferences
None specified.
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
Types of vasculitis, neutrophil primary granules and monocyte lysosomes
Test
Anti Mullerian Hormone
Other Test Request Name
Test Composition
Not applicable
Intended Use
Used for the assessment of ovarian reserve and the prediction of response to controlled ovarian stimulation (COS) in conjunction with other clinical and laboratory findings.
Methodology
Electrochemiluminescence Immunoassay (ECLIA)
Laboratory Section
Immunology
Special Instructions/Patient Preparations
• Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.
Collection/Sample Container
Red or Gold tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
1-3 mL Serum
Alternative Specimen and Volume Requirement
1-3 mL Plasma (Li-Heparin)
Specimen Stability
• Room Temperature (15-25°C)
3 Days
• Refrigerated Temperature (2-8°C)
5 Days
• Freezer Temperature (-20°C)
6 Months (Freeze only once)
Transport Temperature
Transport specimen at 2°C – 8 °C (with cold packs)
Rejection Criteria
• Hemolyzed specimen
• Markedly lipemic sample
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used
• Plasma specimen from EDTA tube
Running Day
Batch Running (7 Days TAT)
Cut Off Time
7:00 PM
TAT/Releasing of Results
ROUTINE (on running days)
• 4 hours after receipt of specimen/ arrival of messenger
STAT (on running days only)
• 2½ hours from extraction/messenger arrival
Reference Interval/Result Interpretation
MALE: 1.43-11.60 ng/mL
FEMALE:
<20Y: No reference range established
20Y & 1 Day - 25Y: 1.66-9.49 ng/mL
25Y & 1 Day - 30Y: 1.88-9.16 ng/mL
30Y & 1 Day - 35Y: 0.67-7.55 ng/mL
35Y & 1 Day - 40Y: 0.78-5.24 ng/mL
40Y & 1 Day - 45Y: 0.10-2.96 ng/mL
45Y & 1 Day - 51Y: 0.05-2.06 ng/mL
Limitations/Interferences
• Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.
• In rare cases, interference due to extremely high titers of antibodies to analyte?specific antibodies, streptavidin or ruthenium can occur.
Frequently Asked Questions (FAQs)
Q : Should every woman of child-bearing years have an AMH test?
A : For most women, the AMH test is not considered necessary unless they are having fertility issues. Most women will not need to have this test performed.
Q : Should every male child have an AMH test?
A : No, testing is not routinely needed. It is only indicated when there are questions about a baby's sexual development.
Related Words/Test
FSH, LH, Inhibin B, Estrogens, Testosterone, Progesterone
Test
Anti Musk
Other Test Request Name
Test Composition
Not Applicable
Intended Use
Myasthenia marker, to be tested for when Anti-acethylcholine Receptor Antibodies are negative (10 to 20% of cases).
For the formation and maintenance of the neuromuscular junction.
Methodology
Enzyme Immunoassay (EIA)
Laboratory Section
Special Test
Special Instructions/Patient Preparations
No patient preparation necessary.
Collection/Sample Container
Red or Gold tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
3 mL Serum
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
24 Hours
• Refrigerated Temperature (2-8°C)
7 Days
• Freezer Temperature (-20°C)
28 Days
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
Running Day
Batch Running
Cut Off Time
Friday, 4:00 PM
TAT/Releasing of Results
1 month after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation
Available upon request
Limitations/Interferences
None specified.
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
Myasthenia marker
Test
Anti NMDA Receptor (CSF)
Other Test Request Name
Test Composition
Not Applicable
Intended Use
Confirm diagnosis of anti NMDAR encephalitis.
Maybe used in monitoring treatment response in individuals who are antibody positive.
Methodology
Immunoflourescence
Laboratory SectionSpecial Test
Special Instructions/Patient Preparations
Collection/Sample Container
Plastic leak proof sterile container (screw cap)
NOTE: Sample must be properly packed (triple packaging)
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
2mLCerebrospinal Fluid (CSF)
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
24 Hours
• Refrigerated Temperature (2-8°C)
7 Days
• Freezer Temperature (-20°C)
28 Days
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
Running Day
Batch running
Cut Off Time
Friday, 4:00 PM
TAT/Releasing of Results
1 month after cut-off (excluding Saturdays, Sundays and Holidays
Reference Interval/Result Interpretation
Available upon request
Limitations/Interferences
None specified
Frequently Asked Questions (FAQs)
Not Applicable
Related Words/Test
Not Applicable
Test
Anti SRP/Myositis Marker
Other Test Request Name
Test Composition
Intended Use
These Antibodies have a cytoplasmic aspect on Hep2 cells (used for Anti nuclear Abs screening). They are associated with a Polymyositis Type including myositis, Raynaud phenomen, lung fibrosis, articular pain and palm hyperkeratosis.
Methodology
Immunodot
Laboratory Section
Not Applicable
Special Instructions/Patient Preparations
No patient preparatiion necessary.
Collection/Sample Container
Red or Gold Tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
3 mL Serum
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
24 Hours
• Refrigerated Temperature (2-8°C)
7 Days
• Freezer Temperature (-20°C)
28 Days
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
Running Day
Batch Running
Cut Off Time
Friday, 12:00 PM
TAT/Releasing of Results
2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation
Not Applicable
Limitations/Interferences
Not Applicable
Frequently Asked Questions (FAQs)
Q: What are the symptoms of myositis?
A:Symptoms of Myositis
Q: How quickly does myositis progress?
A:Polymyositis and dermatomyositis are usually chronic (long-lasting) but typically improve after one to two months of treatment. Inclusion body myositis is also chronic. Since there is no reliably effective treatment for inclusion body myositis, symptoms usually worsen gradually over a period of years.
Q: Does myositis cause tingling?
A:Mild facial weakness may occur in one-third of IBM patients; though there are usually no sensory symptoms, such as numbness and tingling, up to 30% of patients have sensory findings on exam or electrophysiological testing, such as nerve conduction studies as part of an electromyogram (EMG).
Q: What triggers myositis?
A:Myositis means inflammation of the muscles that you use to move your body. An injury, infection, or autoimmune disease can cause it. Two specific kinds are polymyositis and dermatomyositis. Polymyositis causes muscle weakness, usually in the muscles closest to the trunk of your body.
Q: Who gets myositis?
A:Anyone can get myositis, but it usually affects women more than men. Adults between the ages of 30 and 60, and children between the ages of 5 and 15 are more likely to get myositis.
Related Words/Test
Polymyositis, Dermatomyositis, Idiopathic Inflammatory Myopathy
Test
Anti Thyroxine Peroxidase
Other Test Request Name
• Thyroid Antimicrosomal Antibody
• Thyroid Peroxidase Antibody
• Anti-TPO
• Anti-Microsomal Antibodies (AMA)
Test Composition
Not applicable
Intended Use
• Helps diagnose an autoimmune thyroid disease and to distinguish it from other forms of thyroid dysfunction
• Thyroid antibodies are autoantibodies targeted against one or more components on the thyroid. Graves' disease and Hashimoto's thyroiditis are commonly associated with the presence of anti-thyroid autoantibodies.
Methodology
Chemiluminescent Microparticle Immunoassay (CMIA)
Laboratory Section
Immunology
Special Instructions/Patient Preparations
No patient preparation necessary.
Collection/Sample Container
Red or Gold tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
1-3 mL Serum
Alternative Specimen and Volume Requirement
1-3 mL Plasma (Li-heparin, Na-Heparin, EDTA)
Specimen Stability
• Room Temperature (15-25°C)
8 Hours
• Refrigerated Temperature (2-8°C)
72 Hours
• Freezer Temperature (-20°C)
30 Days
Transport Temperature
Transport specimen at 2°C – 8 °C (with cold packs)
Rejection Criteria
• Hemolyzed specimen
• Markedly lipemic specimen
• Specimen stored/transported outside the temperature range
• Quantity not sufficient
• Improperly labeled specimens
Running Day
Monday, Thursday
Cut Off Time
Monday to Saturday:10:00 PM
Sunday:6:00PM
TAT/Releasing of Results
ROUTINE (on running days)
• 4 hours after receipt of specimen/ arrival of messenger
STAT (on running days only)
• 2½ hours from extraction/messenger arrival
Reference Interval/Result Interpretation
0.00-5.61 IU/mL
Limitations/Interferences
• Some specimens may not dilute linearly because of the heterogeneity of the autoantibodies with respect to physiochemical properties.
• Specimens from patients who have received preparations of mouse monoclonal antibodies for diagnosis or therapy may contain human antimouse antibodies (HAMA). Such specimens may show either falsely elevated or depressed values when tested with assay kits that employ mouse monoclonal antibodies.
• Heterophilic antibodies in human serum can react with reagent immunoglobulins, interfering with in vitro immunoassay. The presence of heterophilic antibodies in a patient specimen may cause anomalous values to be observed.
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
Thyroid Antibodies, Thyroid Autoantibodies; Antithyroid Antibodies; Antimicrosomal Antibody; Thyroid Microsomal Antibody; Thyroid Peroxidase Antibody; Thyroperoxidase Antibody; TPO; Anti-TPO; TBII; Antithyroglobulin Antibody; TgAb; TSH Receptor Antibody; TRAb; Thyrotropin Receptor Antibodies; Thyroid Stimulating Immunoglobulin; TSI, Thyroid Peroxidase Antibody; Thyroglobulin Antibody; Thyroid Stimulating Hormone Receptor Antibody
Test
Anti- Gliadin Ab IGG (EIA)
Other Test Request Name
Test Composition
Not applicable
Intended Use
A marker for celiac disease - assayed in parallel to the anti-endomysium, anti-transglutaminase and anti-reticulin antibody test.
Useful for deciding weather or not a jejunal biopsy is warranted. The IgG assay is more sensitive but less specific.
Methodology
Fluorescent enzyme-immunoassay
Laboratory Section
Special Test
Special Instructions/Patient Preparations
No patient preparation necessary.
Collection/Sample Container
Red or Gold tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
3 mL Serum
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
24 Hours
• Refrigerated Temperature (2-8°C)
7 Days
• Freezer Temperature (-20°C)
28 Days
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
Running Day
Batch Running
Cut Off Time
Friday, 4:00 PM
TAT/Releasing of Results
2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation
Available upon request
Limitations/Interferences
None specified.
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
Celiac disease, Anti-tissue Transglutaminase Antibody; tTG; tTGA; Endomysial Antibody; EMA; DGP; ARA; Total IgA, Tissue Transglutaminase Antibody; Deaminated Gliadin Peptide Antibodies; Anti-Endomysial Antibodies; Anti-Reticulin Antibodies; Quantitative Immunoglobulin A
Test
Anti-Smooth Muscle Ab (IF)
Other Test Request Name
Test Composition
Not Applicable
Intended Use
This test detects the presence of antibodies against smooth muscle, which is useful in the diagnosis of type I autoimmune hepatitis (Type I AIH).
Methodology
Indirect Immunofluorescence Test
Laboratory Section
Serology Section
Special Instructions/Patient Preparations
No patient preparation required.
Collection/Sample Container
Plain Red Top or Gold Top
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
2 - 3 mL Serum
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
Not specified
• Refrigerated Temperature (2-8°C)
7 days
• Freezer Temperature (-20°C)
2 weeks
Transport Temperature
Transport specimen at 2 – 10 °C (with cold packs)
Rejection Criteria
Running Day
Wednesday
Cut Off Time
Tuesday, 8:00PM
TAT/Releasing of Results
Thursday, 4:00PM
Reference Interval/Result Interpretation
Significant Level:
Limitations/Interferences
Not applicable.
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
Test
Antibody Screening-TEST TEMPORARILY UNAVAILABLE
Other Test Request Name
Not applicable
Test Composition
Not applicable
Intended Use
Routinely used in conjunction with typing and cross matching before the administration of blood products, especially RBCs, to avoid transfusion reactions and to prevent notably decreased survival of transfused RBCs. It is also used in antenatal screening to detect the presence of antibodies in a pregnant woman's serum that could result in hemolytic disease of the fetus and newborn.
Methodology
Column Agglutination Technology
Laboratory Section
Special Test
Special Instructions/Patient Preparations
No patient preparation required
Collection/Sample Container
1) Plain Red Topand(3 pcs) 4 mL EDTA
Note: Use of 2mL EDTA tube is highly discouraged.
Use of Gel Separator tubes are NOT acceptable
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
3pcs of 4ml EDTA whole blood and 1 Red top whole blood
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
Not Specified
• Refrigerated Temperature (2-8°C)
7 days
• Freezer Temperature (-20°C)
Not Specified
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
Running Day
Monday to Saturday
Cut Off Time
4:00 PM
TAT/Releasing of Results
After 5 days (excluding Saturdays, Sundays and Holiday)
Reference Interval/Result Interpretation
NEGATIVE
Limitations/Interferences
Antibody picture is valid only for 3 days if transfusion is performed.
Frequently Asked Questions (FAQs)
Q: Do I need to have this test done?
A: This is usually performed in patients requiring blood transfusion, patients with suspected transfusion reactions, blood donors and pregnant women.
Related Words/Test
Antibody Identification, Antibody Panel, Pre-transfusion testing, Antenatal Screening, Alloantibodies, Autoantibodies, Cross-matching
Test
APAS Work Up
Other Test Request Name
Test Composition
• Partial Thromboplastin Time
• SCT
• DRVVT
• Anti CardiolipinIgG
• Anti CardiolipinIgM
Intended Use
• For autoimmune disorder that is associated with pregnancy.
• Anti-phospholid Antibodies Syndrome (APAS) Work up is used to determine the cause of improper blood clot formation, recurrent miscarriage, low platelet count and/or prolonged Activated Partial Thromboplastin time (APTT).
Methodology
Multi-wavelength transmitted light method
• DRVVT
• SCT
• PTT
Enzyme Link Immunosorbent Assay (ELISA)
• Anti-Cardiolipin IgM
• Anti-Cardiolipin IgG
Laboratory Section
Hematology
Special Instructions/Patient Preparations
Collection/Sample Container
Two (2) Citrated Blue top
• DRVVT
• SCT
• PTT
One (1) Gold top
• Anti-Cardiolipin IgG
• Anti-Cardiolipin IgM
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
• Plasma: at least 2mL
• Serum: at least 1 mL
Alternative Specimen and Volume Requirement
Not applicable:
• DRVVT
• SCT
• PTT
Red top:
• Anti-Cardiolipin IgG
• Anti-Cardiolipin IgM
Specimen Stability
• Room Temperature (15-25°C)
• PTT: 4 hours
• Refrigerated Temperature (2-8°C)
ACA IgG & ACA IgM: 4days
• Freezer Temperature (-20°C)
7 Days
ACA IgG & ACA IgM: >4days
Transport Temperature
• Anti-Cardiolipin IgG and IgM (Serum) – transport at 2°C to 10°C (with cold packs)
• DRVVT, SCT and PTT (Plasma)– transport at room temperature (15°C to 25°C)
NOTE:
• Sample must be sent to laboratory immediately after collection.
• Samples that cannot be sent to HPD laboratory within 4 hours should be stored at -20°C and should be transported with dry ice.
Rejection Criteria
• Improper use of anticoagulant
• Insufficient volume of blood to anticoagulant (<90% filled)
• Improperly labeled specimens
• Hemolyzed specimens
• Specimen stored and transported outside the temperature requirement
Running Day
Wednesday and Saturday
• Anti-CardiolipinIgG
• Anti-CardiolipinIgM
• DRVV
• SCT
Daily
• PTT
Cut Off Time
Wednesday and Saturday; 2:00 pm
Anti-CardiolipinIgG
Anti-CardiolipinIgM
DRVVT
SCT
Monday up to Saturday, 8:00pm
PTT
Sunday, 4:00 PM
PTT
TAT/Releasing of Results
Thursday and Sunday; 9:00 AM
3 hours after receipt of specimen/ messenger arrival
· PTT
Reference Interval/Result Interpretation
• APTT: 25.00-40.00Seconds
• DRVVT: 0.80-1.20
• SCT: 1.08-1.21
• Anti-Cardiolipin IgG:
NEGATIVE = <12.00 GPL-U/mL
POSITIVE = >18.00 GPL-U/mL
EQUIVOCAL = 12.00 – 18.00 GPL-U/mL
• Anti-Cardiolipin IgM:
NEGATIVE = <12.00 MPL-U/mL
POSITIVE = >18.00 MPL-U/mL
EQUIVOCAL = 12.00 – 18.00 MPL-U/mL
Limitations/Interferences
• Dysfibrinogenemia
• Heparin intake
• Intrinsic pathway deficiencies
• Presence of factor inhibitors
• Hepatic disorders
• Treatments with vitamin K antagonists
• Treatment with thrombin inhibitors
• DIC
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
Lupus Anticoagulant Testing, Cardiolipin Antibodies, Partial Thromboplastin Time (PTT, aPTT), Beta-2 Glycoprotein 1 Antibodies, Antinuclear Antibody (ANA), Autoimmune Diseases, Excessive Clotting Disorders, Antiphospholipid Syndrome, Lupus, Pregnancy.
| Test | Apolipoprotein B |
| Other Test Request Name |
|
| Test Composition | Not Applicable |
| Intended Use | These lipid (cholesterol, triglycerides and phospholipids) transport proteins represent the main constituent of Low Density Lipoprotein particles. High levels of this apolipoprotein B concomitant with low levels of apolipoprotein A1 constitute a strong risk factor for atherosclerosis. Apolipoprotein B-100 (also called apolipoprotein B or apo B) is a protein that is involved in the metabolism of lipids and is the main protein constituent of lipoproteins such as very low-density lipoprotein (VLDL) and low-debsity lipoprotein (LDL, the "bad cholesterol"). This test measures the amount of apo B in the blood. |
| Methodology | Immuno turbidimetry |
| Laboratory Section | Special Test |
| Special Instructions/Patient Preparations | Fasting 8-12 Hours. |
| Collection/Sample Container | Red or Gold Top |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | 3 mL Serum |
| Alternative Specimen and Volume Requirement | Not Applicable |
| Specimen Stability | |
| • Room Temperature (15-25°C) | 24 Hours |
| • Refrigerated Temperature (2-8°C) | 7 Days |
| • Freezer Temperature (-20°C) | 28 Days |
| Transport Temperature | Transport specimen at 2 – 8 °C (with cold packs) |
| Rejection Criteria |
|
| Running Day | Batch Running |
| Cut Off Time | Friday, 4:00 PM |
| TAT/Releasing of Results | 2 weeks after (excluding Saturdays, Sundays and Holidays) |
| Reference Interval/Result Interpretation | Available upon request |
| Limitations/Interferences | None specified |
| Frequently Asked Questions (FAQs) | Q: Why get tested? A:To help evaluate your risk of developing cardiobascular disease (CVD); sometimes to help monitor treatment for high cholesterol or to help diagnose a rare inherited apolipoprotein B (apo B) deficiency Q: When to get tested? A:When you have a personal or family history of heart disease and/or high cholesterol and triglyceride levels and your healthcare provider is trying to determine your risk of developing CVD; sometimes on a regular basis when you are being treated for high cholesterol; rarely when your healthcare practitioner suspects that you have an inherited apo B deficiency Q: When is it ordered? A:Apo B may be measured, along with an apo A-or other lipid tests,when a healthcare practitioner is trying to evaluate someone's risk of developing (CVD) and when a person has a personal or family history of heart disease and/or abnormal lipid levels, especially when the person has significantly elevated triglyceride levels. Sometimes apo B is ordered to monitor a person who is undergoing treatment for high cholesterol. Q: What can I do to lower my apo B? A:Diet and exercise changes that lower LDL levels (and increase HDL, the "good" cholesterol) will lower your apo B levels and decrease your risk of heart disease. |
| Related Words/Test | Evaluate the risk for cardiovascular disease, Lipid Panel, LDL Cholesterol, Cholesterol, Triglycerides, HDL Cholesterol, Apo A-I, Lipoprotein A, High-sensitivity C-reactive Protein (hs-CRP), Homocysteine, Cardiac Risk Assessment, |
Test
Apolipoprotein E Genotype
Other Test Request Name
Test Composition
Not applicable
Intended Use
This test determines the subtypes of apoe which will aid in the risk assessment of corornary heart disease (CHD) and hyperlipoproteinemia.
Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
Laboratory Section
Special Test
Special Instructions/Patient Preparations
No patient preparation necessary.
Collection/Sample Container
EDTA or Violet TubeSpecimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
2 pcs of 4 mL EDTA whole blood
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
24 Hours
• Refrigerated Temperature (2-8°C)
7 Days
• Freezer Temperature (-20°C)
28 Days
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
Running Day
Batch Running
Cut Off Time
Monday, 12:00 PM
TAT/Releasing of Results
2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation
Not Applicable
Limitations/Interferences
None specified
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
APOE Cardiac Risk, Apolipoprotein E Genotyping, Atherosclerosis,
hypercholesterolemi